Viviane de Cassia Pereira1*, Edson Rodrigues-Filho2, Enzo Monte Canedo2, Ana Carolina Alves dos Santos2, Patrícia Umeda Grisi1, Maristela Imatomi1 and Sonia Cristina Juliano Gualtieri1*
1 Programa de Pós-Graduação em Ecologia e Recursos Naturais, Universidade Federal de São Carlos, Via Washington Luís, km 235, CP 676, CEP 13565-905,
São Carlos, SP, Brazil
2 Programa de Pós-Graduação em Química, Universidade Federal de São Carlos, Via Washington Luís, km 235, CP 676, CEP 13565-905, São Carlos, SP, Brazil.
Serjania lethalis A.St.-Hil is a species of liana from the Brazilian savanna (cerrado) that belongs to the family Sapindaceae. In this study, we evaluated the phytotoxic profile of young and mature leaves of Serjania lethalis by assaying inhibition of growth of wheat coleoptile (Triticum aestivum L.). The bioactive fractions were fractionated by medium pressure chromatography and the subfractions obtained from this process were assayed over again. Phytotoxic subfractions were analyzed by NMR and LC-MS techniques. Subfractions obtained from ethanolic extracts of young leaves exhibited a pronounced phytotoxic effect when compared to others, inhibiting the growth of wheat coleoptile. Spectroscopic analysis led to the identification of polymeric procyanidin, procyanidin oligomers (tannins) and a saponin.
Keywords: Phytotoxicity, Procyanidins, Saponin, Tannins
RECEIVED: October 14, 2015
ACCEPTED November 13, 2015